|
|
|
|
Hela Cells were stained
with Mitotracker red (red), Hoescht (blue) and transfected with EGFP linked
to FLJ10618 (green) - a putative mitochondrial deoxypyrimitine transporter.
The cells were imaged alive and rendered with Imaris to demonstrate the
protein is localized in mitochondria (work done by: Drs. Karen M. King,
Mark F. Vickers and Carol E. Cass).
|
|
|
The Cell Imaging Facility at Cross Cancer Institute is a multi-user resource
that enables researchers in the Institute and in the University of Alberta
to implement imaging techniques in their research. The Facility focuses
on applications of advanced, light microscopic imaging techniques (live
cell imaging, FRAP, FLIM, FRET, Fluorescence Correlative Spectroscopy
(FCS), etc.) in various aspects of Cancer/Cell biology research.
The Facility was established
in 1998. Various light microscopic imaging instruments are available for
local researchers (including confocal, multi-photon, deconvolution, Laser
catapulting and high throughput FISH system, etc.). It is supported by
CIHR (Canadian Institute of Health Research) and Alberta Cancer Board.
Various research projects are currently conducted in the Facility. Briefly,
main focuses of the department are: 1) Tumor biology; 2) DND damage and
repair; 2) Cell cycle control and nucleus structure; 3) Role of transporter
proteins in nucleoside biology and therapeutics.
Bitplane software
has been a major imaging analysis tool for the Facility. Beside routine
3-D visualization with the software, Bitplane software enables us to conduct
many complex image analyses which are impossible to do without it (e.g.
4-d tracking; co-localization analysis, quantifying DNA damage using image
based analysis, etc.).
|