|
|
|
|
Volume-rendered views
of wild-type motor axons, synapses, and muscles. Neurons in C155; GFP
larvae were labeled with anti-GFP (green), and muscles are labeled with
rhodamine- phalloidin (red). These image shows the actual paths of the
nerves through the three- dimensional muscle field viewed from the exterior
of the ISNb, ISNd and the ventrolateral and ventral muscles. The cloudy
cell at the upper right is an unidentified cell type that also expresses
GFP in this driver line.
Image: Rachel Kraut,
Cyrus Papan |
|
|
The research at Scott Fraser's Biological Imaging Center explores the
patterning of cell lineages, cell migrations and axonal connections during
vertebrate embryogenesis. The goal is to develop new imaging techniques
and experimental strategies that permit single-cell resolution studies
of each of these key processes in intact developing embryos. Given that
there is no single imaging technology or developmental model system that
is ideal for all studies, we employ a parallel approach; we both consider
various systems such as the frog, chicken or mouse and use techniques
as diverse as video, laser scanning confocal, laser scanning two-photon,
or magnetic resonance microscopy.
|
